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Protein purification scientist skills for your resume and career
15 protein purification scientist skills for your resume and career
1. Western Blotting
- Aided in the development of a system that utilized LICOR technologies for Western Blotting experiments within the company.
- Demonstrated target engagement in target issues by western blotting.
2. HPLC
- Utilized HPLC to quantify product through experimentation leading to identification of start material changes and anticipated manufacturing results.
- Experienced in antibody purification via column chromatography, FITC conjugation, fractionation, fragmentation, and use of HPLC.
3. Elisa
An enzyme-linked immunosorbent assay or ELISA is an examination or test to measure and detect a person's specific antigen, antibodies, and protein. This type of test will identify if the sample component is infected with a relative disease or condition such as HIV infection, anemia, Zika Virus, and Lyme disease with just a single experiment.
- Experienced scientist in standardizing ELISA assays for existing and synthetic molecules in cancer therapy for Personalized Drug Management.
- Utilized Elisa, fluorescence polarization assay to identify the target.
4. ION Exchange
- Worked with different types of Sepharose resins, including Protein G, A, DEAE ion exchange, and IgM.
- Simplified ion exchange chromatography process from two step to single step wash process.
5. Cell Culture
- Isolated DNA from cell culture to determine possible genetic markers of osteoarthritis pain which lead to a novel target opportunity.
- Mentored junior scientists in cell culture technology and cellular biology and provided support in troubleshooting, and atypical issue resolution.
6. Laboratory Equipment
- Performed lab managerial tasks such as hematology analyzers QC/maintenance, as well as maintaining the calibration schedule for laboratory equipment.
- Perform laboratory equipment qualification, calibration, preventative maintenance and coordinates outside contractor support for the same.
7. GMP
GMP stands for Good Manufacturing Practice. It is a system that ensures that all products like food, beverages, and medicinal drugs that are produced comply with the quality standards. It helps in minimizing the risks and hazards that cannot be eliminated after the testing of final products.
- Project and personnel management for large scale GMP downstream purification of plant made pharmaceuticals.
- Developed and scaled-up manufacturing processes for liquid consumer products from laboratory to final GMP production scale at various external manufacturing sites.
8. UV
Ultraviolet (UV) radiation is a form of non-ionizing radiation emitted by the sun and other human-made sources such as tanning beds. UV radiation falls under three primary categories: ultraviolet A (UVA), ultraviolet B (UVB), and ultraviolet C (UVC).
- Provided OSLO based first-order ray tracing optical design-analysis of lenses-crystal elements for UV, Visible, IR.
- Developed UV curable optically clear adhesives for glass composite bonding.
9. Electrophoresis
- Developed assays to detect residual complement activity Performed SDS-PAGE and acid urea gel electrophoresis.
- Support analyst for ICP OES, Potting and Cross Sectioning, Micro-hardness testing, FTIR, and Capillary Electrophoresis.
10. Buffer Preparation
- Support functions such as pH, conductivity, cleaning, set-up and CIP of equipment, component weigh-outs and buffer preparation.
11. Crystallography
- Carried out Limited proteolysis of helicase protein in order to determine potential stable constructs for Crystallography.
12. Profiling
- Performed statistical and spectral analysis profiling of EEG waveform activity.
- Perform metabolic profiling experiments, data processing and project reporting.
13. GLP
GLP stands for "good laboratory practice." This refers to the integrity and quality measures that non-clinical laboratories have in place to ensure accurate research and market testing. GLP is most often used in pharmaceutical companies where new drugs must be tested before being approved for sale, but the practice can also be used in food packaging and preparation test centers.
- Performed routine laboratory assignments in accordance with company SOP's and any other applicable guidelines and regulations including GLP if applicable.
- Managed laboratory operations in Life Sciences department including acquisition, calibration, and maintenance of equipment that produced GLP compliance.
14. NMR
The Nuclear Magnetic Resonance technique allows observing magnetic fields around an object. The NMR equipment requires monitoring and ensuring their correct and safe utilization.
- Purified triple labelled protein to support NMR studies as an alternative to obtain structural information and understand protein dynamics.
15. ISO
- Completed all relevant paperwork according to specific ISO regulations and guidelines for IVD.
- Directed ISO 9000 4.10 team registration activities and performed internal audits.
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List of protein purification scientist skills to add to your resume

The most important skills for a protein purification scientist resume and required skills for a protein purification scientist to have include:
- Western Blotting
- HPLC
- Elisa
- ION Exchange
- Cell Culture
- Laboratory Equipment
- GMP
- UV
- Electrophoresis
- Buffer Preparation
- Crystallography
- Profiling
- GLP
- NMR
- ISO
- Affinity Chromatography
- Extraction
- Product Development
- FDA
- Imac
- R
- IND
- Enzymes
- Therapeutic Proteins
- Target Proteins
- BCA
Updated January 8, 2025