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Top 50 Research Biochemist Skills

Below we've compiled a list of the most important skills for a Research Biochemist. We ranked the top skills based on the percentage of Research Biochemist resumes they appeared on. For example, 22.3% of Research Biochemist resumes contained Assay Development as a skill. Let's find out what skills a Research Biochemist actually needs in order to be successful in the workplace.

These are the most important skills for a Research Biochemist:

1. Assay Development

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high Demand
Here's how Assay Development is used in Research Biochemist jobs:
  • Produced proteins in large scale for structure determination, HTS screens and in-vitro assay developments.

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2. Protein

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high Demand
Here's how Protein is used in Research Biochemist jobs:
  • Designed and developed a novel cell pure filter aid for large scale protein purification and production for pharmaceutical and industrial applications.
  • Characterize purified proteins with respect to biological function and response to small molecule inhibitors.
  • Developed purification and characterization methods related to plant- and microbe-derived protein analysis and degradation.
  • Extracted proteins from plant tissues and screened extracts for anti-microbial activity against agricultural pathogens.
  • Design cloning, expression and purification protocols for therapeutically important proteins.
  • Developed medium throughput parallel protein purification protocol used in multiple projects.
  • Purified various proteins for protein structure determination and functional studies.
  • Managed X-ray crystallography data collection facility and protein crystallization lab.
  • Optimized protein expression using Piccolo & Sonata automation systems.
  • Determined protein crystal structures for projects with DuPont Crop Protection and DuPont Central Research and Development.
  • Subject matter expert for aggregation and fragmentation assays for all protein, vaccine and antibody projects.
  • Screened target proteins and their variants for selecting high yield clones.
  • Established a protein characterization and proteomics platform in the Corn Host Pathogen Resistance Research Unit (CHPRRU).
  • Studied the effects of AKT inhibitors on the AKT/TCL1 protein interactions and on TCL1- dependent tumorigenesis.
  • Investigated neural activation of Unfolded Protein Response (UPR) genes after anesthesia or hypoxia.
  • Analyze protein samples using Light Scattering and Mass Spectrometry.
  • Group leader for Waters UPLC troubleshooting * Support bioprocess development; Biophysical characterization; Protein and vaccine stability tests
  • Ethanol-induced Translocation of cAMP-dependent Protein Kinase to the Nucleus: Mechanism and Functional Consequences.
  • Key Achievements: * Authored technical reports, DOPs, SOPs for multiple protein products.
  • Research skills are: Utilize AKTA FPLC and AKTAxpress (Amersham Biosciences) to perform protein purifications.

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3. Hplc

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high Demand
Here's how Hplc is used in Research Biochemist jobs:
  • Designed HPLC protein purification procedure to produce high purity protein to identify new chemical entities in a critical assay.
  • Conducted TNT extraction efficiency experiments using HPLC methodology.
  • Developed both a radioactive TCA precipitation and HPLC assays for the Insulin Receptor Activator program to quantify bound vs. unbound substrate.
  • Ensured quality of purified protein by SEC-HPLC, SDS-PAGE and LAL analysis.
  • General laboratory techniques - HPLC, dissolution APP 2, APP 4, DSC, TGA.
  • Set up and developed isocratic and gradient HPLC methods, system suitability, method validation and data manipulation/presentation.
  • Developed and validated HPLC methods for antibiotics in fluids and tissues to support metabolism and pharmacokinetics trials.
  • Performed calibration and preventative maintenance of pH meter, Spec, HPLC, and Oligo Synthesizer.
  • Prepared buffer solutions for HPLC machines and reagents for oligo synthesis.
  • Performed HPLC determination of serum thiamine using fluoremetric detection and serum retinol using UV detection including liquid/liquid and solid/liquid extraction.
  • Analyzed the oligo quality using Spectrophotometry and HPLC.

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4. DNA

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Here's how DNA is used in Research Biochemist jobs:
  • Developed and validated method and process for preparation of water-soluble self-assembly DNA-CNT hybrid.
  • Investigated DNA / protein homologies between species using DNA analysis software.
  • Collaborated in the design and analysis of preclinical Molecular Profiling experiments including DNA microarrays for project support.
  • Determined the structure of post-translational protein, known as ubiquitin, and its related functions (signaling DNA replication repair).
  • Performed recombinant DNA cloning in E.coli bacteria.
  • Invented a method using diatomaceous earth (DE) as a filter aid for purifications of plasmid DNA and proteins.
  • Sub-cloned and end-labeled neuropeptide cDNAs for use as templates for RNA probes in the ribonuclease protection assay.
  • Developed cDNA synthesis kits using the RNase-H minus reverse-transcriptase, SuperScript.

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5. Rt-Pcr

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Here's how Rt-Pcr is used in Research Biochemist jobs:
  • Performed RT-PCR assay development and validation.
  • Determined serotype of putative viral infections in clinical samples utilizing RNA extraction and RT-PCR sequencing techniques.
  • Established the qRT-PCR assay for quantitating Flu genomes and showed comparable results to a plaque assay.

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6. Biochemistry Support

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7. Cell Culture

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8. Elisa

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Here's how Elisa is used in Research Biochemist jobs:
  • Developed, validated and implemented biochemical, enzyme, ELISA and cell-based assays as primary and secondary screening assays.
  • Evaluated compounds for cancer research programs in the cell-based ECL and ELISA assays.
  • Test immune responses of clinical sample (body fluids) using ELISA.
  • Developed ELISA for detection of insulin from adult stem cells using Apolco kit.
  • Employed assay formats such as ELISA, TR/FRET, SPA, radioligand binding, and others.

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9. Data Analysis

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10. RNA

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Here's how RNA is used in Research Biochemist jobs:
  • Organized monthly journal reviews and disseminated information across team.
  • Isolated RNA for molecular profiling for the identification of new small molecule targets in Diabetes and Obesity.
  • Identified alternate vendors of lab consumables resulting in savings of approximately $40,000.00 per year.
  • Presented research at the International Association for Dental Research Conference in Miami.
  • Published in top-tier journals including first author on high-impact, well-cited articles.
  • Acknowledged for expert technical assistance in journals of biochemical research, 1994-1996.
  • Participated in internal and external research groups to present findings.
  • Developed microarray facility and SQL databases for internal projects and departmental collaborations.
  • Collaborated with SSL International plc.
  • Co-ordinated data acquisition in collaboration with a CRO for DP1 expression profiling in 72 human tissues and at the mRNA level.
  • Studied hormonal regulation of animal cell gene expression Purified and characterized yeast mitochondrial RNA polymerase

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11. FDA

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Here's how FDA is used in Research Biochemist jobs:
  • Prompt method development of protein aggregation assays, which enabled process and formulation groups to make FDA approved products.
  • Coordinated IQ/OQ/PQ testing for new equipment and maintenance of PQ testing as per FDA guidelines.
  • Worked with compounds from all stages of FDA required testing for subsequent approval.
  • Composed written reports for the FDA and Clinical Department.

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12. High Throughput

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Here's how High Throughput is used in Research Biochemist jobs:
  • Managed, developed and performed high throughput compound screens to identify and characterize leads for various GPCR targets.
  • Developed cell-based assays to validate therapeutic compounds for human metabolic disorders with high throughput screenings.

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13. Biology

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Here's how Biology is used in Research Biochemist jobs:
  • Managed a biochemistry/molecular biology laboratory and supervised research associates.
  • Performed nucleic acid extraction and molecular biology techniques to isolate gene sequences of Air Force operational interest.
  • Provided technical leadership in structural biology to DuPont research community.
  • Coordinated chemistry/ biology groups representing different projects, which resulted in the development of novel leads.
  • Supported the molecular biology group by developing systems encompassing mass production of a polyhedrosis virus, bacterial and fungal pathogens.
  • level scientists to provide disease area-specific biology support
  • Conducted basic recombinant molecular biology techniques.

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14. HTS

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Here's how HTS is used in Research Biochemist jobs:
  • Established 1536-well format biochemical and cell-based assay; analyzed screening data with software used in HTS data analyses.
  • Trained/supervised new employees, industrial trainees, temporary contractors in all aspects of HTS and data management.
  • Handled development of high throughput screens (HTS) in the New Lead Discovery department.
  • Developed/tested the results databases in a large scale HTS department.

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15. Research Projects

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Here's how Research Projects is used in Research Biochemist jobs:
  • Acquired Skills: Management: Designed and supervised independent research projects.

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16. Small Molecules

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Here's how Small Molecules is used in Research Biochemist jobs:
  • Processed, analyzed and uploaded HTS data that led to the discovery of new structural classes of small molecules.

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17. HPV

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Here's how HPV is used in Research Biochemist jobs:
  • Evaluated 2000 samples in 4 HPV type-specific assays to assess antibody relative to HN-Cancer status study age and gender matched subjects.
  • Aligned with internal clinical, IT and operational groups to coordinate testing across HPV vaccine trials and programs.
  • Maintained QC and import of HPV PCR Data from all HPV vaccine trials to clinical databases.
  • Constructed AAV2 vector for a new vaccine vector discovery and vectors for HPV vaccine discovery.
  • Collaborated with National Cancer Institute for transfer of HPV Neutralization Assay.
  • Served as Key-contact for Merck: Harvard School of Public Health A collaborative research on HPV in Head and Neck Cancer.
  • Planned and executed the re- optimization of 15 HPV PCR Assays for use on the selected Stratagene MX3005P instrument platform.
  • Authored and executed PCR Assay Validation Protocols for HPV PCR assays on the Stratagene MX3005P instrument.
  • Authored, reviewed and revised SOPs for HPV Laboratory Procedures.
  • Demonstrated cross-protection potential of Quadrivalent HPV vaccine, Gardasil(TM).

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18. Sds-Page

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19. Clinical Trials

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Here's how Clinical Trials is used in Research Biochemist jobs:
  • Managed inventory of antigens of staphylococcus for clinical trials.
  • Researched ingredients, dosages, clinical trials, and suppliers.
  • Develop appropriate immunological methods testing ongoing clinical trials (vaccine projects).

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20. QC

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Here's how QC is used in Research Biochemist jobs:
  • Developed and implemented QC tests ensuring assay capability of prototype instruments.
  • Automated enzyme methods development and validation for QC and research purposes.
  • Conducted manufacturing support, training and troubleshooting for plant QC/QA laboratories.

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21. GLP

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Here's how GLP is used in Research Biochemist jobs:
  • Study Director for regulatory studies requiring detailed GLP protein analysis for submission to Global regulatory agencies.
  • Performed toxicokinetic calculations and Co-authorized toxicokinetic reports Planned, conducted and analyzed PK ADME studies in accordance with GLP

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22. Oversaw

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23. Study Protocol

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24. Blot

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Here's how Blot is used in Research Biochemist jobs:
  • Assessed new technologies for Piccolo process improvement for high throughput assay to replace western blots.
  • Optimized protein expression and analyzed products with Western blots.
  • Analyzed expressed protein samples using Western blots/Image quant and Caliper assay.

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25. NMR

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Here's how NMR is used in Research Biochemist jobs:
  • Labeled proteins for Bio NMR.

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26. SAR

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Here's how SAR is used in Research Biochemist jobs:
  • Identified lead compounds enabling medicinal chemists to develop structure activity relationships (SAR).
  • Generated IC50 data for use in SAR and lead identification.
  • Performed mutagenic analysis of adrenergic receptor subtypes to clarify SAR.

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27. Basic Research

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Here's how Basic Research is used in Research Biochemist jobs:
  • Provided expertise to support the transformation of a basic research laboratory to a regulated environment.
  • Supported basic research by providing high quality purified monoclonal antibodies from mammalian, hybridoma culture for preclinical development and assay development.

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28. Clinical Samples

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Here's how Clinical Samples is used in Research Biochemist jobs:
  • Demonstrated development of resistance to antiviral agents through analysis of clinical samples in support of lead optimization and early clinical development.
  • Authored summary reports of method validation activities, clinical summary reports and memos documenting experimental analysis of Phase I clinical samples.

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29. HIV

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Here's how HIV is used in Research Biochemist jobs:
  • Constructed a serial of HIV expression vectors containing different promoters and expressed in different cell lines.
  • Worked with live wild type and mutant HIV (virus).
  • Served as assistant internal quality control and archive manager* Authored and reviewed standard operating procedures (SOPs)
  • Conducted recombinant DNA technology studies to support both HIV and Hepatitis A Vaccine programs.

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30. Target Identification

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Here's how Target Identification is used in Research Biochemist jobs:
  • Supported target identification and validation.
  • Provided autoradiography in rodent brains, which contributed to target identification and validation.
  • Provided GPCR ligand binding assays (cells or membranes) which supported target identification and validation, and drug development.
  • Provided expertise in autoradiography and ligand binding assays in the studies of target identification, validation and drug development.

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31. Animal Studies

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Here's how Animal Studies is used in Research Biochemist jobs:
  • Work with basic research to design and facilitate animal studies.
  • Purified second generation HPV by Ion exchange, reverse phase and size exclusion chromatography for animal studies.

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32. R

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Here's how R is used in Research Biochemist jobs:
  • Performed ELISA assay platform optimization, validation, and transfer assessments to maximize quality and quantity of clinical trial data reporting.
  • Supervised personnel within the Sample Processing group, providing leadership, direction, and employee development.
  • Set up batches of 8000 samples/week in CTMS system for processing by PCR assay team.
  • Reported in 5 journal publications, Karl T. Kelsey as Sr.
  • Reported in 2 research journal publication, Janine Bryan as Sr.
  • Experience in building and managing database for clinical samples.
  • Led the experimental execution of biomarker development and validation in a set of experimental medicine studies for atherosclerosis programs.
  • Processed peripheral atherosclerotic plaque powders to optimize extraction conditions.
  • Experience conducting investigational QA projects.
  • Qualified reagents, antigens, and proprietary pre-manufactured antigen coated plates to insure optimal performance in ELISA assays.
  • Designed excel program to effectively organize and analyze IC50 data for in vitro and animal screens.
  • Trained new analysts in the use and interpretation of multiple assays and computer and analytical systems.
  • Evaluated cell lines for their ability to express neuropeptides by using multiplex PCR.
  • Assisted in the maintenance, repair, and trouble shooting of analytical equipment.
  • Used in binding assays and cell transfections.
  • Coordinated and conducted animal experiments with Laboratory Animal Resources to examine the pharmacokinetics of zaragozic acids in rhesus monkeys.
  • Measured of areas of stroke damage with immunohistochemistry, in-situ hybridization and TUNEL.
  • Handled, organized, and tested human clinical sera and other biologics in a regulated environment for a multifaceted client base.
  • Reported in the 6 journal publications, Kathrin Jansen or Eliav Barr as Senior Authors.
  • Provided early involvement and targeted support for clinical identification of biomarkers involving gene expression profiling, proteomics, and immunohistochemistry.

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33. Therapeutic Areas

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Here's how Therapeutic Areas is used in Research Biochemist jobs:
  • Generated, analyzed and reported all results to the therapeutic areas and the on-site data base.

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34. PCC

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Here's how PCC is used in Research Biochemist jobs:
  • Established a primary hepatocyte assay to monitor lipid formation using radio-labeled lipid precursors to support preclinical candidate (PCC) selection.

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35. GMP

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Here's how GMP is used in Research Biochemist jobs:
  • Prepared assay reagents and QC samples in GMP-regulated BSL-2 scale-up manufacturing facility.
  • Developed and designed new cleaning procedures and executed them for GMP equipment.
  • Scheduled GMP activity and coordinated testing and cleaning of the area.
  • Performed cGMP audit on electronic laboratory notebooks.
  • Key Responsibilities: Manufacture phase 1 clinical material under cGMP conditions.
  • Managed and maintained department labs, storage and cGMP facilities.
  • Process Lead for manufacture of four cGMP cell bank campaigns.
  • Purified native and recombinant T. gondii cGMP-dependent protein kinases and determined their biochemical and kinetic properties to support target validation studies.

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36. E.Coli

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37. NIH

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Here's how NIH is used in Research Biochemist jobs:
  • Grant Writing: Regular submission of grants to funding agencies, including NIH and the Department of Defense.
  • Assisted in preparation of NIH and UC grants, developed intranet-website, supervised lab personnel.

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38. Powerpoint

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Here's how Powerpoint is used in Research Biochemist jobs:
  • Prepared conference posters and PowerPoint presentations for senior management.

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39. Research Associates

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Here's how Research Associates is used in Research Biochemist jobs:
  • Supervised 3 research associates for scientific and personal development.
  • Led research associates in the crystallization of precursor elastin and connective tissue characterization under federal government grants.

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40. Analytical Methods

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Here's how Analytical Methods is used in Research Biochemist jobs:
  • Developed immunological assay to study cardiac regulation and used bioanalytical methods to perform structural studies.
  • Developed analytical methods (LC/MS/MS) for bi-directional transport analysis.
  • Develop, qualify, and validate analytical methods for protein follow-on biologics.

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41. Lims

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42. Cancer Patients

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43. Data Management

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44. Hazardous Materials

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Here's how Hazardous Materials is used in Research Biochemist jobs:
  • Trained staff on safety compliance as division's primary responder to hazardous materials.

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45. New Technologies

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Here's how New Technologies is used in Research Biochemist jobs:
  • Evaluated, developed, and introduced new technologies for protein analysis and separation.

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46. Body Fluids

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47. Pichia

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Here's how Pichia is used in Research Biochemist jobs:
  • Purified novel, agriculturally-useful enzymes from fungal (Pichia) culture supernatants for protein sequence determination.
  • Purified His-Tag protein from Pichia with affinity chromatography.
  • Research Biochemist Expressed proteins in insect cells, E.Coli and Pichia.

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48. IEX

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49. HIC

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Here's how HIC is used in Research Biochemist jobs:
  • Participated in several programs which have concluded with a successful progression into clinical trials.
  • Developed assays for HPSEC-UV-MALS-FL, high throughput assays, MacroIMS, DLS, HPLC, HIC, preservatives, and detergents.
  • Prompt coordination of outsourcing of RNA, total carbohydrate and lipids assays to Lancaster Labs, which facilitated product approval.
  • Established and supervised a new biochemical pharmacology laboratory, which was the milestone of the company research strategy goals.
  • Conducted crystallographic and modeling study of key enzyme GDH and reactivation enzyme in the bio-PDO synthetic pathway.
  • Helped the toxicology group by maintaining mammalian and invertebrate tissue culture techniques, and determining the cytopathic effects of toxic substances.
  • Coordinated testing for Gardasil vaccine, which led to prompt product launch and qualification of new manufacturing facilities.
  • Automated HPLC testing for more than ten antibody projects, which reduced results turnaround time three-fold.
  • Created audit map for analytical testing, which helped efficient HPV regulatory filing.
  • Purified anti-microbial proteins from active plant extracts using chromatographic and electrophoretic techniques.

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50. Cros

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Here's how Cros is used in Research Biochemist jobs:
  • Facilitated and trained laboratory technicians on malaria microscopy clinical diagnosis sponsored by African Malaria Network (AMANET).
  • Generated, analyzed, reported, and coordinated data cross-departmentally with animal research and medicinal chemistry.
  • Interacted with cross-functional teams to facilitate lead advancement.
  • Help designing scaffolds / microspheres for tissue engineering lab.
  • Determined antibody cross-reactivity to blood cell components and tissues by immunocytochemical techniques.
  • Utilized immunohistochemical and fluorescence confocal microscopy techniques to study the expression and regulation of the key enzymes of the mannitol cycle.

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Research Biochemist Jobs

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20 Most Common Skills For A Research Biochemist

Assay Development

29.2%

Protein

17.6%

Hplc

6.7%

DNA

4.7%

Rt-Pcr

4.2%

Biochemistry Support

3.8%

Cell Culture

3.6%

Elisa

3.3%

Data Analysis

3.1%

RNA

2.9%

FDA

2.7%

High Throughput

2.5%

Biology

2.2%

HTS

2.0%

Research Projects

2.0%

Small Molecules

2.0%

HPV

2.0%

Sds-Page

2.0%

Clinical Trials

1.8%

QC

1.6%
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Typical Skill-Sets Required For A Research Biochemist

Rank Skill
1 Assay Development 22.3%
2 Protein 13.4%
3 Hplc 5.1%
4 DNA 3.6%
5 Rt-Pcr 3.2%
6 Biochemistry Support 2.9%
7 Cell Culture 2.7%
8 Elisa 2.6%
9 Data Analysis 2.4%
10 RNA 2.2%
11 FDA 2.0%
12 High Throughput 1.9%
13 Biology 1.7%
14 HTS 1.5%
15 Research Projects 1.5%
16 Small Molecules 1.5%
17 HPV 1.5%
18 Sds-Page 1.5%
19 Clinical Trials 1.4%
20 QC 1.2%
21 GLP 1.2%
22 Oversaw 1.2%
23 Study Protocol 1.2%
24 Blot 1.0%
25 NMR 1.0%
26 SAR 1.0%
27 Basic Research 1.0%
28 Clinical Samples 1.0%
29 HIV 0.9%
30 Target Identification 0.9%
31 Animal Studies 0.9%
32 R 0.9%
33 Therapeutic Areas 0.9%
34 PCC 0.7%
35 GMP 0.7%
36 E.Coli 0.7%
37 NIH 0.7%
38 Powerpoint 0.7%
39 Research Associates 0.7%
40 Analytical Methods 0.7%
41 Lims 0.7%
42 Cancer Patients 0.7%
43 Data Management 0.7%
44 Hazardous Materials 0.7%
45 New Technologies 0.7%
46 Body Fluids 0.7%
47 Pichia 0.5%
48 IEX 0.5%
49 HIC 0.5%
50 Cros 0.5%
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